dc.contributor.author | Han, Sewoon | |
dc.contributor.author | Shin, Yoojin | |
dc.contributor.author | Jeong, Hyo Eun | |
dc.contributor.author | Jeon, Jessie S. | |
dc.contributor.author | Huh, Dongeun | |
dc.contributor.author | Sohn, Lydia L. | |
dc.contributor.author | Chung, Seok | |
dc.contributor.author | Kamm, Roger Dale | |
dc.date.accessioned | 2016-01-19T17:51:17Z | |
dc.date.available | 2016-01-19T17:51:17Z | |
dc.date.issued | 2015-12 | |
dc.date.submitted | 2015-10 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/100923 | |
dc.description.abstract | The construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally. | en_US |
dc.description.sponsorship | Korea (South). Ministry of Science, ICT and Future Planning (National Research Foundation of Korea. Pioneer Research Center Program (NRF-2015M3C1A3002152) | en_US |
dc.description.sponsorship | Korea (South). Ministry of Science, ICT and Future Planning (BioNano Health-Guard Research Center Global Frontier Project H-GUARD_2014M3A6B2060524) | en_US |
dc.language.iso | en_US | |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/srep18290 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nature Publishing Group | en_US |
dc.title | Constructive remodeling of a synthetic endothelial extracellular matrix | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Han, Sewoon, Yoojin Shin, Hyo Eun Jeong, Jessie S. Jeon, Roger D. Kamm, Dongeun Huh, Lydia L. Sohn, and Seok Chung. “Constructive Remodeling of a Synthetic Endothelial Extracellular Matrix.” Scientific Reports 5 (December 21, 2015): 18290. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Mechanical Engineering | en_US |
dc.contributor.mitauthor | Shin, Yoojin | en_US |
dc.contributor.mitauthor | Kamm, Roger Dale | en_US |
dc.relation.journal | Scientific Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Han, Sewoon; Shin, Yoojin; Jeong, Hyo Eun; Jeon, Jessie S.; Kamm, Roger D.; Huh, Dongeun; Sohn, Lydia L.; Chung, Seok | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7232-304X | |
mit.license | OPEN_ACCESS_POLICY | en_US |