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dc.contributor.authorHan, Sewoon
dc.contributor.authorShin, Yoojin
dc.contributor.authorJeong, Hyo Eun
dc.contributor.authorJeon, Jessie S.
dc.contributor.authorHuh, Dongeun
dc.contributor.authorSohn, Lydia L.
dc.contributor.authorChung, Seok
dc.contributor.authorKamm, Roger Dale
dc.date.accessioned2016-01-19T17:51:17Z
dc.date.available2016-01-19T17:51:17Z
dc.date.issued2015-12
dc.date.submitted2015-10
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/100923
dc.description.abstractThe construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally.en_US
dc.description.sponsorshipKorea (South). Ministry of Science, ICT and Future Planning (National Research Foundation of Korea. Pioneer Research Center Program (NRF-2015M3C1A3002152)en_US
dc.description.sponsorshipKorea (South). Ministry of Science, ICT and Future Planning (BioNano Health-Guard Research Center Global Frontier Project H-GUARD_2014M3A6B2060524)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep18290en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleConstructive remodeling of a synthetic endothelial extracellular matrixen_US
dc.typeArticleen_US
dc.identifier.citationHan, Sewoon, Yoojin Shin, Hyo Eun Jeong, Jessie S. Jeon, Roger D. Kamm, Dongeun Huh, Lydia L. Sohn, and Seok Chung. “Constructive Remodeling of a Synthetic Endothelial Extracellular Matrix.” Scientific Reports 5 (December 21, 2015): 18290.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorShin, Yoojinen_US
dc.contributor.mitauthorKamm, Roger Daleen_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHan, Sewoon; Shin, Yoojin; Jeong, Hyo Eun; Jeon, Jessie S.; Kamm, Roger D.; Huh, Dongeun; Sohn, Lydia L.; Chung, Seoken_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7232-304X
mit.licenseOPEN_ACCESS_POLICYen_US


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