Improving d-glucaric acid production from myo-inositol in E. coli by increasing MIOX stability and myo-inositol transport
Author(s)Shiue, Eric; Prather, Kristala L
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d-glucaric acid has been explored for a myriad of potential uses, including biopolymer production and cancer treatment. A biosynthetic route to produce d-glucaric acid from glucose has been constructed in Escherichia coli ( Moon et al., 2009b), and analysis of the pathway revealed myo-inositol oxygenase (MIOX) to be the least active enzyme. To increase pathway productivity, we explored protein fusion tags for increased MIOX solubility and directed evolution for increased MIOX activity. An N-terminal SUMO fusion to MIOX resulted in a 75% increase in d-glucaric acid production from myo-inositol. While our directed evolution efforts did not yield an improved MIOX variant, our screen isolated a 941 bp DNA fragment whose expression led to increased myo-inositol transport and a 65% increase in d-glucaric acid production from myo-inositol. Overall, we report the production of up to 4.85 g/L of d-glucaric acid from 10.8 g/L myo-inositol in recombinant E. coli.
DepartmentMassachusetts Institute of Technology. Department of Chemical Engineering
Shiue, Eric, and Kristala L.J. Prather. “Improving d-Glucaric Acid Production from Myo-Inositol in E. Coli by Increasing MIOX Stability and Myo-Inositol Transport.” Metabolic Engineering 22 (March 2014): 22–31.
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