High-throughput functional genomics using CRISPR–Cas9
Author(s)Shalem, Ophir; Zhang, Feng; Sanjana, Neville
MetadataShow full item record
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MIT
Nature Reviews Genetics
Nature Publishing Group
Shalem, Ophir, Neville E. Sanjana, and Feng Zhang. “High-Throughput Functional Genomics Using CRISPR–Cas9.” Nature Reviews Genetics 16, no. 5 (April 9, 2015): 299–311.
Author's final manuscript