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High-throughput functional genomics using CRISPR–Cas9

Author(s)
Shalem, Ophir; Zhang, Feng; Sanjana, Neville
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Abstract
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges.
Date issued
2015-04
URI
http://hdl.handle.net/1721.1/102585
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MIT
Journal
Nature Reviews Genetics
Publisher
Nature Publishing Group
Citation
Shalem, Ophir, Neville E. Sanjana, and Feng Zhang. “High-Throughput Functional Genomics Using CRISPR–Cas9.” Nature Reviews Genetics 16, no. 5 (April 9, 2015): 299–311.
Version: Author's final manuscript
ISSN
1471-0056
1471-0064

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