| dc.contributor.author | Shalem, Ophir | |
| dc.contributor.author | Zhang, Feng | |
| dc.contributor.author | Sanjana, Neville | |
| dc.date.accessioned | 2016-05-23T00:03:50Z | |
| dc.date.available | 2016-05-23T00:03:50Z | |
| dc.date.issued | 2015-04 | |
| dc.identifier.issn | 1471-0056 | |
| dc.identifier.issn | 1471-0064 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/102585 | |
| dc.description.abstract | Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges. | en_US |
| dc.description.sponsorship | Klarman Family Foundation (Fellowship) | en_US |
| dc.description.sponsorship | Massachusetts Institute of Technology. Simons Center for the Social Brain (Postdoctoral Fellowship) | en_US |
| dc.description.sponsorship | National Human Genome Research Institute (U.S.) (K99-HG008171) | en_US |
| dc.description.sponsorship | National Institute of Mental Health (U.S.) (DP1-MH100706) | en_US |
| dc.description.sponsorship | National Institute of Neurological Disorders and Stroke (U.S.) (R01-NS07312401) | en_US |
| dc.description.sponsorship | National Science Foundation (U.S.) (Waterman Award) | en_US |
| dc.description.sponsorship | W. M. Keck Foundation | en_US |
| dc.description.sponsorship | Damon Runyon Cancer Research Foundation | en_US |
| dc.description.sponsorship | Kinship Foundation. Searle Scholars Program | en_US |
| dc.description.sponsorship | Klingenstein Foundation | en_US |
| dc.description.sponsorship | Vallee Foundation | en_US |
| dc.description.sponsorship | Merkin Foundation | en_US |
| dc.description.sponsorship | Simons Foundation | en_US |
| dc.description.sponsorship | New York Stem Cell Foundation | en_US |
| dc.language.iso | en_US | |
| dc.publisher | Nature Publishing Group | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1038/nrg3899 | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
| dc.source | PMC | en_US |
| dc.title | High-throughput functional genomics using CRISPR–Cas9 | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Shalem, Ophir, Neville E. Sanjana, and Feng Zhang. “High-Throughput Functional Genomics Using CRISPR–Cas9.” Nature Reviews Genetics 16, no. 5 (April 9, 2015): 299–311. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
| dc.contributor.department | McGovern Institute for Brain Research at MIT | en_US |
| dc.contributor.mitauthor | Shalem, Ophir | en_US |
| dc.contributor.mitauthor | Sanjana, Neville | en_US |
| dc.contributor.mitauthor | Zhang, Feng | en_US |
| dc.relation.journal | Nature Reviews Genetics | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Shalem, Ophir; Sanjana, Neville E.; Zhang, Feng | en_US |
| dspace.embargo.terms | N | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0003-2782-2509 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
