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Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics

Author(s)
Sarkar, Aniruddh; Hou, Han Wei; Mahan, Alison E.; Han, Jongyoon; Alter, Galit
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Abstract
Isolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required for many diagnostic, therapeutic and research applications. Current affinity-based protein or cell separation methods use binary ‘bind-elute’ separations and are inefficient when applied to the isolation of multiple low-abundance proteins or cell types. We present a method for rapid and multiplexed, yet inexpensive, affinity-based isolation of both proteins and cells, using a size-coded mixture of multiple affinity-capture microbeads and an inertial microfluidic particle sorter device. In a single binding step, different targets–cells or proteins–bind to beads of different sizes, which are then sorted by flowing them through a spiral microfluidic channel. This technique performs continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding. We demonstrate the simultaneous isolation of multiple antibodies from serum and multiple cell types from peripheral blood mononuclear cells or whole blood. We use the technique to isolate low abundance antibodies specific to different HIV antigens and rare HIV-specific cells from blood obtained from HIV+ patients.
Date issued
2016-03
URI
http://hdl.handle.net/1721.1/103075
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Ragon Institute of MGH, MIT and Harvard
Journal
Scientific Reports
Publisher
Springer Nature
Citation
Sarkar, Aniruddh, Han Wei Hou, Alison. E. Mahan, Jongyoon Han, and Galit Alter. “Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics.” Scientific Reports 6 (March 30, 2016): 23589.
Version: Final published version
ISSN
2045-2322

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