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dc.contributor.authorSarkar, Aniruddh
dc.contributor.authorHou, Han Wei
dc.contributor.authorMahan, Alison E.
dc.contributor.authorHan, Jongyoon
dc.contributor.authorAlter, Galit
dc.date.accessioned2016-06-09T14:11:29Z
dc.date.available2016-06-09T14:11:29Z
dc.date.issued2016-03
dc.date.submitted2015-12
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/103075
dc.description.abstractIsolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required for many diagnostic, therapeutic and research applications. Current affinity-based protein or cell separation methods use binary ‘bind-elute’ separations and are inefficient when applied to the isolation of multiple low-abundance proteins or cell types. We present a method for rapid and multiplexed, yet inexpensive, affinity-based isolation of both proteins and cells, using a size-coded mixture of multiple affinity-capture microbeads and an inertial microfluidic particle sorter device. In a single binding step, different targets–cells or proteins–bind to beads of different sizes, which are then sorted by flowing them through a spiral microfluidic channel. This technique performs continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding. We demonstrate the simultaneous isolation of multiple antibodies from serum and multiple cell types from peripheral blood mononuclear cells or whole blood. We use the technique to isolate low abundance antibodies specific to different HIV antigens and rare HIV-specific cells from blood obtained from HIV+ patients.en_US
dc.description.sponsorshipUnited States. Defense Advanced Research Projects Agency (DARPA Dialysis-like Therapy (DLT) program under SSC Pacific N66001-11-1-4182)en_US
dc.description.sponsorshipBill & Melinda Gates Foundationen_US
dc.language.isoen_US
dc.publisherSpringer Natureen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep23589en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleMultiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidicsen_US
dc.typeArticleen_US
dc.identifier.citationSarkar, Aniruddh, Han Wei Hou, Alison. E. Mahan, Jongyoon Han, and Galit Alter. “Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics.” Scientific Reports 6 (March 30, 2016): 23589.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentRagon Institute of MGH, MIT and Harvarden_US
dc.contributor.mitauthorSarkar, Aniruddhen_US
dc.contributor.mitauthorHou, Han Weien_US
dc.contributor.mitauthorMahan, Alison E.en_US
dc.contributor.mitauthorHan, Jongyoonen_US
dc.contributor.mitauthorAlter, Galiten_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSarkar, Aniruddh; Hou, Han Wei; Mahan, Alison. E.; Han, Jongyoon; Alter, Galiten_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-1570-9445
dc.identifier.orcidhttps://orcid.org/0000-0001-7215-1439
dc.identifier.orcidhttps://orcid.org/0000-0003-4284-8467
mit.licenseOPEN_ACCESS_POLICYen_US


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