Efficient CRISPR-Cas9 mediated gene disruption in primary erythroid progenitor cells

Author(s)
Li, Hojun; Shi, Jiahai; Huang, Nai-Jia; Natarajan, Anirudh; Eng, Jennifer C.; ... Show more
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Abstract
The study of isolated primary progenitor cells offers great insight into developmental biology and human disease. In particular, ex vivo culture of isolated primary erythroid progenitor cells replicates the differentiation events that occur during in vivo erythropoiesis. Herein we report a high-efficiency method for CRISPR-Cas9 mediated gene disruption in isolated primary erythroid progenitor cells. We use this method to generate the novel result that Lmna is required in terminal erythroid differentiation.
Date issued
2016-06
URI
http://hdl.handle.net/1721.1/106569
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology
Journal
Haematologica
Publisher
European Hematology Association (EHA)/Ferrata Storti Foundation
Citation
Li, H., J. Shi, N.-J. Huang, N. Pishesha, A. Natarajan, J. C. Eng, and H. F. Lodish. “Efficient CRISPR-Cas9 Mediated Gene Disruption in Primary Erythroid Progenitor Cells.” Haematologica 101, no. 6 (March 11, 2016): e216-e219.
Version: Author's final manuscript
ISSN
0390-6078
1592-8721
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