| dc.contributor.author | Li, Hojun | |
| dc.contributor.author | Shi, Jiahai | |
| dc.contributor.author | Huang, Nai-Jia | |
| dc.contributor.author | Natarajan, Anirudh | |
| dc.contributor.author | Eng, Jennifer C. | |
| dc.contributor.author | Pishesha, Novalia | |
| dc.contributor.author | Lodish, Harvey F | |
| dc.date.accessioned | 2017-01-20T19:50:30Z | |
| dc.date.available | 2017-01-20T19:50:30Z | |
| dc.date.issued | 2016-06 | |
| dc.identifier.issn | 0390-6078 | |
| dc.identifier.issn | 1592-8721 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/106569 | |
| dc.description.abstract | The study of isolated primary progenitor cells offers great insight into developmental biology and human disease. In particular, ex vivo culture of isolated primary erythroid progenitor cells replicates the differentiation events that occur during in vivo erythropoiesis. Herein we report a high-efficiency method for CRISPR-Cas9 mediated gene disruption in isolated primary erythroid progenitor cells. We use this method to generate the novel result that Lmna is required in terminal erythroid differentiation. | en_US |
| dc.description.sponsorship | Frederick Lovejoy (Research Grant) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (grant NIH/NHLBI 2 P01 HL032262-25) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | European Hematology Association (EHA)/Ferrata Storti Foundation | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.3324/haematol.2015.135723 | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
| dc.source | Prof. Lodish via Courtney Crummett | en_US |
| dc.title | Efficient CRISPR-Cas9 mediated gene disruption in primary erythroid progenitor cells | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Li, H., J. Shi, N.-J. Huang, N. Pishesha, A. Natarajan, J. C. Eng, and H. F. Lodish. “Efficient CRISPR-Cas9 Mediated Gene Disruption in Primary Erythroid Progenitor Cells.” Haematologica 101, no. 6 (March 11, 2016): e216-e219. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
| dc.contributor.approver | Lodish, Harvey F | en_US |
| dc.contributor.mitauthor | Pishesha, Novalia | |
| dc.contributor.mitauthor | Lodish, Harvey F | |
| dc.relation.journal | Haematologica | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Li, H.; Shi, J.; Huang, N.-J.; Pishesha, N.; Natarajan, A.; Eng, J. C.; Lodish, H. F. | en_US |
| dspace.embargo.terms | N | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0001-9306-8271 | |
| dc.identifier.orcid | https://orcid.org/0000-0002-7029-7415 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
