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Comparison of Cas9 activators in multiple species

Author(s)
Chavez, Alejandro; Tuttle, Marcelle; Pruitt, Benjamin W; Ewen-Campen, Ben; Chari, Raj; Ter-Ovanesyan, Dmitry; Haque, Sabina J; Cecchi, Ryan J; Kowal, Emma J K; Buchthal, Joanna; Housden, Benjamin E; Perrimon, Norbert; Church, George; Collins, James J.; ... Show more Show less
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.

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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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Abstract
Several programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.
Date issued
2016-05
URI
http://hdl.handle.net/1721.1/106662
Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science; Massachusetts Institute of Technology. Synthetic Biology Center; Massachusetts Institute of Technology. Department of Biological Engineering
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
.Chavez, Alejandro et al. “Comparison of Cas9 Activators in Multiple Species.” Nature Methods 13.7 (2016): 563–567.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105

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