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Comparison of Cas9 activators in multiple species

dc.contributor.authorChavez, Alejandro
dc.contributor.authorTuttle, Marcelle
dc.contributor.authorPruitt, Benjamin W
dc.contributor.authorEwen-Campen, Ben
dc.contributor.authorChari, Raj
dc.contributor.authorTer-Ovanesyan, Dmitry
dc.contributor.authorHaque, Sabina J
dc.contributor.authorCecchi, Ryan J
dc.contributor.authorKowal, Emma J K
dc.contributor.authorBuchthal, Joanna
dc.contributor.authorHousden, Benjamin E
dc.contributor.authorPerrimon, Norbert
dc.contributor.authorChurch, George
dc.contributor.authorCollins, James J.
dc.date.accessioned2017-01-27T20:45:31Z
dc.date.available2017-01-27T20:45:31Z
dc.date.issued2016-05
dc.date.submitted2016-01
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttp://hdl.handle.net/1721.1/106662
dc.description.abstractSeveral programmable transcription factors exist based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems, and we assess the role of cooperativity in maximizing gene expression.en_US
dc.description.sponsorshipUnited States. Defense Threat Reduction Agency (Grant HDTRA1-14-1-0006)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nmeth.3871en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleComparison of Cas9 activators in multiple speciesen_US
dc.typeArticleen_US
dc.identifier.citation.Chavez, Alejandro et al. “Comparison of Cas9 Activators in Multiple Species.” Nature Methods 13.7 (2016): 563–567.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Synthetic Biology Centeren_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.mitauthorCollins, James J.
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsChavez, Alejandro; Tuttle, Marcelle; Pruitt, Benjamin W; Ewen-Campen, Ben; Chari, Raj; Ter-Ovanesyan, Dmitry; Haque, Sabina J; Cecchi, Ryan J; Kowal, Emma J K; Buchthal, Joanna; Housden, Benjamin E; Perrimon, Norbert; Collins, James J; Church, Georgeen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-5560-8246
mit.licensePUBLISHER_POLICYen_US


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