Virtual microfluidics for digital quantification and single-cell sequencing
Author(s)
Xu, Liyi; Brito, Ilana Lauren; Alm, Eric J; Blainey, Paul C
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Show full item recordAbstract
We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions.
Date issued
2016-08Department
Massachusetts Institute of Technology. Department of Biological EngineeringJournal
Nature Methods
Publisher
Nature Publishing Group
Citation
Xu, Liyi et al. “Virtual Microfluidics for Digital Quantification and Single-Cell Sequencing.” Nature Methods 13.9 (2016): 759–762.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105