| dc.contributor.author | Xu, Liyi | |
| dc.contributor.author | Brito, Ilana Lauren | |
| dc.contributor.author | Alm, Eric J | |
| dc.contributor.author | Blainey, Paul C | |
| dc.date.accessioned | 2017-02-23T18:21:59Z | |
| dc.date.available | 2017-02-23T18:21:59Z | |
| dc.date.issued | 2016-08 | |
| dc.date.submitted | 2016-02 | |
| dc.identifier.issn | 1548-7091 | |
| dc.identifier.issn | 1548-7105 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/107124 | |
| dc.description.abstract | We have developed hydrogel-based virtual microfluidics as a simple and robust alternative to complex engineered microfluidic systems for the compartmentalization of nucleic acid amplification reactions. We applied in-gel digital multiple displacement amplification (dMDA) to purified DNA templates, cultured bacterial cells and human microbiome samples in the virtual microfluidics system, and demonstrated whole-genome sequencing of single-cell MDA products with excellent coverage uniformity and markedly reduced chimerism compared with products of liquid MDA reactions. | en_US |
| dc.description.sponsorship | Broad Institute of MIT and Harvard (Lawrence Summers Fellowship) | en_US |
| dc.description.sponsorship | Burroughs Wellcome Fund (Career Award) | en_US |
| dc.description.sponsorship | Massachusetts Institute of Technology. Center for Microbiome Informatics and Therapeutics | en_US |
| dc.description.sponsorship | National Human Genome Research Institute (U.S.) (Broad Institute of MIT and Harvard. Grant U54HG003067) | en_US |
| dc.description.sponsorship | Massachusetts Institute of Technology. Center for Environmental Health Sciences | en_US |
| dc.description.sponsorship | Fiji. Ministry of Health | en_US |
| dc.language.iso | en_US | |
| dc.publisher | Nature Publishing Group | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1038/nmeth.3955 | en_US |
| dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
| dc.source | PMC | en_US |
| dc.title | Virtual microfluidics for digital quantification and single-cell sequencing | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Xu, Liyi et al. “Virtual Microfluidics for Digital Quantification and Single-Cell Sequencing.” Nature Methods 13.9 (2016): 759–762. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
| dc.contributor.mitauthor | Xu, Liyi | |
| dc.contributor.mitauthor | Brito, Ilana Lauren | |
| dc.contributor.mitauthor | Alm, Eric J | |
| dc.contributor.mitauthor | Blainey, Paul C | |
| dc.relation.journal | Nature Methods | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Xu, Liyi; Brito, Ilana L; Alm, Eric J; Blainey, Paul C | en_US |
| dspace.embargo.terms | N | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0002-6699-2634 | |
| dc.identifier.orcid | https://orcid.org/0000-0001-8294-9364 | |
| dc.identifier.orcid | https://orcid.org/0000-0001-7014-3830 | |
| mit.license | PUBLISHER_POLICY | en_US |
