Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy
Author(s)Scarcelli, Giuliano; Patel, Kripa; Polacheck III, William Joseph; Tavakoli Nia, Hadi; Grodzinsky, Alan J; Kamm, Roger Dale; Yun, Seok Hyun (Andy); ... Show more Show less
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Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.
DepartmentHarvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Mechanical Engineering
Nature Publishing Group
Scarcelli, Giuliano et al. “Noncontact Three-Dimensional Mapping of Intracellular Hydromechanical Properties by Brillouin Microscopy.” Nature Methods 12.12 (2015): 1132–1134.
Author's final manuscript