Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy

Author(s)
Scarcelli, Giuliano; Patel, Kripa; Polacheck III, William Joseph; Tavakoli Nia, Hadi; Grodzinsky, Alan J; ... Show more
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Abstract
Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.
Date issued
2015-10
URI
http://hdl.handle.net/1721.1/107616
Department
Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Mechanical Engineering
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Scarcelli, Giuliano et al. “Noncontact Three-Dimensional Mapping of Intracellular Hydromechanical Properties by Brillouin Microscopy.” Nature Methods 12.12 (2015): 1132–1134.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105
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