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Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy

dc.contributor.authorScarcelli, Giuliano
dc.contributor.authorPatel, Kripa
dc.contributor.authorPolacheck III, William Joseph
dc.contributor.authorTavakoli Nia, Hadi
dc.contributor.authorGrodzinsky, Alan J
dc.contributor.authorKamm, Roger Dale
dc.contributor.authorYun, Seok Hyun (Andy)
dc.date.accessioned2017-03-21T14:16:22Z
dc.date.available2017-03-21T14:16:22Z
dc.date.issued2015-10
dc.date.submitted2015-03
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttp://hdl.handle.net/1721.1/107616
dc.description.abstractCurrent measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grants P41-EB015903 and R33 CA174550-01)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Grant CBET-0853773)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nmeth.3616en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleNoncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopyen_US
dc.typeArticleen_US
dc.identifier.citationScarcelli, Giuliano et al. “Noncontact Three-Dimensional Mapping of Intracellular Hydromechanical Properties by Brillouin Microscopy.” Nature Methods 12.12 (2015): 1132–1134.en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorPolacheck III, William Joseph
dc.contributor.mitauthorTavakoli Nia, Hadi
dc.contributor.mitauthorGrodzinsky, Alan J
dc.contributor.mitauthorKamm, Roger Dale
dc.contributor.mitauthorYun, Seok Hyun (Andy)
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsScarcelli, Giuliano; Polacheck, William J; Nia, Hadi T; Patel, Kripa; Grodzinsky, Alan J; Kamm, Roger D; Yun, Seok Hyunen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-2728-0746
dc.identifier.orcidhttps://orcid.org/0000-0003-1970-9901
dc.identifier.orcidhttps://orcid.org/0000-0002-4942-3456
dc.identifier.orcidhttps://orcid.org/0000-0002-7232-304X
mit.licensePUBLISHER_POLICYen_US


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