Cas9 gRNA engineering for genome editing, activation and repression
Author(s)Chavez, Alejandro; Tuttle, Marcelle; Chari, Raj; Ter-Ovanesyan, Dmitry; Qian, Jason; Pruitt, Benjamin W; Buchthal, Joanna; Church, George; Kiani, Samira; Hall, Richard N.; Beal, Jacob S; Vora, Suhani Deepak; Kowal, Emma J.; Ebrahimkhani, Mohammad Reza; Collins, James J.; Weiss, Ron; ... Show more Show less
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We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
DepartmentMIT Synthetic Biology Center; Massachusetts Institute of Technology. Department of Biological Engineering
Nature Publishing Group
Kiani, Samira, Alejandro Chavez, Marcelle Tuttle, Richard N Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, et al. “Cas9 gRNA Engineering for Genome Editing, Activation and Repression.” Nature Methods 12, no. 11 (September 7, 2015): 1051–1054.
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