Cas9 gRNA engineering for genome editing, activation and repression

Author(s)
Chavez, Alejandro; Tuttle, Marcelle; Chari, Raj; Ter-Ovanesyan, Dmitry; Qian, Jason; ... Show more
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Abstract
We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.
Date issued
2015-09
URI
http://hdl.handle.net/1721.1/107914
Department
MIT Synthetic Biology Center; Massachusetts Institute of Technology. Department of Biological Engineering
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Kiani, Samira, Alejandro Chavez, Marcelle Tuttle, Richard N Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, et al. “Cas9 gRNA Engineering for Genome Editing, Activation and Repression.” Nature Methods 12, no. 11 (September 7, 2015): 1051–1054.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105
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