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Cas9 gRNA engineering for genome editing, activation and repression

dc.contributor.authorChavez, Alejandro
dc.contributor.authorTuttle, Marcelle
dc.contributor.authorChari, Raj
dc.contributor.authorTer-Ovanesyan, Dmitry
dc.contributor.authorQian, Jason
dc.contributor.authorPruitt, Benjamin W
dc.contributor.authorBuchthal, Joanna
dc.contributor.authorChurch, George
dc.contributor.authorKiani, Samira
dc.contributor.authorHall, Richard N.
dc.contributor.authorBeal, Jacob S
dc.contributor.authorVora, Suhani Deepak
dc.contributor.authorKowal, Emma J.
dc.contributor.authorEbrahimkhani, Mohammad Reza
dc.contributor.authorCollins, James J.
dc.contributor.authorWeiss, Ron
dc.date.accessioned2017-04-06T19:57:41Z
dc.date.available2017-04-06T19:57:41Z
dc.date.issued2015-09
dc.date.submitted2015-04
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttp://hdl.handle.net/1721.1/107914
dc.description.abstractWe demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.en_US
dc.description.sponsorshipNational Human Genome Research Institute (U.S.) (P50 HG005550)en_US
dc.description.sponsorshipUnited States. Department of Energy (DE-FG02-02ER63445)en_US
dc.description.sponsorshipWyss Institute for Biologically Inspired Engineeringen_US
dc.description.sponsorshipUnited States. Army Research Office (DARPA W911NF-11-2-0054)en_US
dc.description.sponsorshipNational Science Foundation (U.S.)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (5R01CA155320-04)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (P50 GM098792)en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (5T32CA009216-34)en_US
dc.description.sponsorshipMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.description.sponsorshipHarvard Medical School. Department of Geneticsen_US
dc.description.sponsorshipDefense Threat Reduction Agency (DTRA) (HDTRA1-14-1-0006)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nmeth.3580en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleCas9 gRNA engineering for genome editing, activation and repressionen_US
dc.typeArticleen_US
dc.identifier.citationKiani, Samira, Alejandro Chavez, Marcelle Tuttle, Richard N Hall, Raj Chari, Dmitry Ter-Ovanesyan, Jason Qian, et al. “Cas9 gRNA Engineering for Genome Editing, Activation and Repression.” Nature Methods 12, no. 11 (September 7, 2015): 1051–1054.en_US
dc.contributor.departmentMIT Synthetic Biology Centeren_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.mitauthorKiani, Samira
dc.contributor.mitauthorHall, Richard N.
dc.contributor.mitauthorBeal, Jacob S
dc.contributor.mitauthorVora, Suhani Deepak
dc.contributor.mitauthorKowal, Emma J.
dc.contributor.mitauthorEbrahimkhani, Mohammad Reza
dc.contributor.mitauthorCollins, James J.
dc.contributor.mitauthorWeiss, Ron
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsKiani, Samira; Chavez, Alejandro; Tuttle, Marcelle; Hall, Richard N; Chari, Raj; Ter-Ovanesyan, Dmitry; Qian, Jason; Pruitt, Benjamin W; Beal, Jacob; Vora, Suhani; Buchthal, Joanna; Kowal, Emma J K; Ebrahimkhani, Mohammad R; Collins, James J; Weiss, Ron; Church, Georgeen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-1736-0937
dc.identifier.orcidhttps://orcid.org/0000-0002-1663-5102
dc.identifier.orcidhttps://orcid.org/0000-0002-5560-8246
dc.identifier.orcidhttps://orcid.org/0000-0003-0396-2443
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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