Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production
Author(s)Li, Zhengjun; Qiao, Kangjian; Liu, Nian; Stephanopoulos, Gregory
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Strains of Yarrowia lipolytica were engineered to express the poly-3-hydroxybutyrate (PHB) biosynthetic pathway. The genes for β-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase, and PHB synthase were cloned and inserted into the chromosome of Y. lipolytica. In shake flasks, the engineered strain accumulated PHB to 1.50 and 3.84% of cell dry weight in complex medium supplemented with glucose and acetate as carbon source, respectively. In fed-batch fermentation using acetate as sole carbon source, 7.35 g/l PHB (10.2% of cell dry weight) was produced. Selection of Y. lipolytica as host for PHB synthesis was motivated by the fact that this organism is a good lipids producer, which suggests robust acetyl-CoA supply also the precursor of the PHB pathway. Acetic acid could be supplied by gas fermentation, anaerobic digestion, and other low-cost supply route.
Journal of Industrial Microbiology & Biotechnology
Li, Zheng-Jun, Kangjian Qiao, Nian Liu, and Gregory Stephanopoulos. “Engineering Yarrowia Lipolytica for Poly-3-Hydroxybutyrate Production.” Journal of Industrial Microbiology & Biotechnology 44, no. 4–5 (November 8, 2016): 605–612.
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