dc.contributor.author | Li, Zhengjun | |
dc.contributor.author | Qiao, Kangjian | |
dc.contributor.author | Liu, Nian | |
dc.contributor.author | Stephanopoulos, Gregory | |
dc.date.accessioned | 2017-05-24T19:20:08Z | |
dc.date.available | 2017-09-03T05:00:05Z | |
dc.date.issued | 2016-11 | |
dc.date.submitted | 2016-11 | |
dc.identifier.issn | 1367-5435 | |
dc.identifier.issn | 1476-5535 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/109321 | |
dc.description.abstract | Strains of Yarrowia lipolytica were engineered to express the poly-3-hydroxybutyrate (PHB) biosynthetic pathway. The genes for β-ketothiolase, NADPH-dependent acetoacetyl-CoA reductase, and PHB synthase were cloned and inserted into the chromosome of Y. lipolytica. In shake flasks, the engineered strain accumulated PHB to 1.50 and 3.84% of cell dry weight in complex medium supplemented with glucose and acetate as carbon source, respectively. In fed-batch fermentation using acetate as sole carbon source, 7.35 g/l PHB (10.2% of cell dry weight) was produced. Selection of Y. lipolytica as host for PHB synthesis was motivated by the fact that this organism is a good lipids producer, which suggests robust acetyl-CoA supply also the precursor of the PHB pathway. Acetic acid could be supplied by gas fermentation, anaerobic digestion, and other low-cost supply route. | en_US |
dc.description.sponsorship | Tsinghua University (Beijing, China). School of Life Sciences | en_US |
dc.description.sponsorship | United States. Department of Energy (DE-SC0008744) | en_US |
dc.description.sponsorship | National Natural Science Foundation (China) (21476014 and 31100025) | en_US |
dc.publisher | Springer-Verlag | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1007/s10295-016-1864-1 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | Springer Berlin Heidelberg | en_US |
dc.title | Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Li, Zheng-Jun, Kangjian Qiao, Nian Liu, and Gregory Stephanopoulos. “Engineering Yarrowia Lipolytica for Poly-3-Hydroxybutyrate Production.” Journal of Industrial Microbiology & Biotechnology 44, no. 4–5 (November 8, 2016): 605–612. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | |
dc.contributor.mitauthor | Li, Zhengjun | |
dc.contributor.mitauthor | Qiao, Kangjian | |
dc.contributor.mitauthor | Liu, Nian | |
dc.contributor.mitauthor | Stephanopoulos, Gregory | |
dc.relation.journal | Journal of Industrial Microbiology & Biotechnology | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2017-04-28T04:50:11Z | |
dc.language.rfc3066 | en | |
dc.rights.holder | Society for Industrial Microbiology and Biotechnology | |
dspace.orderedauthors | Li, Zheng-Jun; Qiao, Kangjian; Liu, Nian; Stephanopoulos, Gregory | en_US |
dspace.embargo.terms | N | en |
dc.identifier.orcid | https://orcid.org/0000-0002-1164-8692 | |
dc.identifier.orcid | https://orcid.org/0000-0001-6909-4568 | |
mit.license | OPEN_ACCESS_POLICY | en_US |