Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning
Author(s)
Ozkumur, Ayca Yalcin; Love, John C; Goods, Brittany A.
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Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV.
Date issued
2015-09Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemical Engineering; Koch Institute for Integrative Cancer Research at MITJournal
Small
Publisher
Wiley Blackwell
Citation
Ozkumur, Ayca Yalcin; Goods, Brittany A. and Love, J. Christopher. “Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.” Small 11, no. 36 (June 2015): 4643–4650 © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Version: Author's final manuscript
ISSN
1613-6810
1613-6829