A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

Author(s)
Lee, Kevin S.; Lee, Harry L. T.; Mozdzierz, Nicholas Joseph; Love, Kerry R.; Shah, Kartik A; ... Show more
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Abstract
We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.
Date issued
2015-05
URI
http://hdl.handle.net/1721.1/109453
Department
Massachusetts Institute of Technology. Center for Biomedical Innovation; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Research Laboratory of Electronics; Koch Institute for Integrative Cancer Research at MIT
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry, The
Citation
Mozdzierz, Nicholas J.; Love, Kerry R.; Lee, Kevin S.; Lee, Harry L. T.; Shah, Kartik A.; Ram, Rajeev J. and Love, J. Christopher. “A Perfusion-Capable Microfluidic Bioreactor for Assessing Microbial Heterologous Protein Production.” Lab on a Chip 15, no. 14 (May 2015): 2918–2922 © 2015 Royal Society of Chemistry
Version: Author's final manuscript
ISSN
1473-0197
1473-0189
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