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A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production

dc.contributor.authorLee, Kevin S.
dc.contributor.authorLee, Harry L. T.
dc.contributor.authorMozdzierz, Nicholas Joseph
dc.contributor.authorLove, Kerry R.
dc.contributor.authorShah, Kartik A
dc.contributor.authorRam, Rajeev J
dc.contributor.authorLove, John C
dc.date.accessioned2017-05-31T14:09:27Z
dc.date.available2017-05-31T14:09:27Z
dc.date.issued2015-05
dc.date.submitted2015-04
dc.identifier.issn1473-0197
dc.identifier.issn1473-0189
dc.identifier.urihttp://hdl.handle.net/1721.1/109453
dc.description.abstractWe present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.en_US
dc.description.sponsorshipUnited States. Defense Advanced Research Projects Agency (N66001-13-C-4025)en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (P30-CA14051)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (2T32GM008334-26)en_US
dc.language.isoen_US
dc.publisherRoyal Society of Chemistry, Theen_US
dc.relation.isversionofhttp://dx.doi.org/10.1039/c5lc00443hen_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleA perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein productionen_US
dc.typeArticleen_US
dc.identifier.citationMozdzierz, Nicholas J.; Love, Kerry R.; Lee, Kevin S.; Lee, Harry L. T.; Shah, Kartik A.; Ram, Rajeev J. and Love, J. Christopher. “A Perfusion-Capable Microfluidic Bioreactor for Assessing Microbial Heterologous Protein Production.” Lab on a Chip 15, no. 14 (May 2015): 2918–2922 © 2015 Royal Society of Chemistryen_US
dc.contributor.departmentMassachusetts Institute of Technology. Center for Biomedical Innovationen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Research Laboratory of Electronicsen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorMozdzierz, Nicholas Joseph
dc.contributor.mitauthorLove, Kerry R.
dc.contributor.mitauthorShah, Kartik A
dc.contributor.mitauthorRam, Rajeev J
dc.contributor.mitauthorLove, John C
dc.relation.journalLab on a Chipen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsMozdzierz, Nicholas J.; Love, Kerry R.; Lee, Kevin S.; Lee, Harry L. T.; Shah, Kartik A.; Ram, Rajeev J.; Love, J. Christopheren_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-0420-2235
dc.identifier.orcidhttps://orcid.org/0000-0003-0921-3144
mit.licenseOPEN_ACCESS_POLICYen_US


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