One-step optogenetics with multifunctional flexible polymer fibers
Author(s)Jia, Xiaoting; Grena, Benjamin; Park, Seongjun; Guo, Yuanyuan; Choe, Han Kyoung; Grena, Benjamin Jean-Baptiste; Kang, Jeewoo; Park, Jiyeon; Lu, Chi; Canales, Andres; Chen, Ritchie; Yim, Yeong Shin; Choi, Bohyun Gloria; Fink, Yoel; Anikeeva, Polina Olegovna; ... Show more Show less
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Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities in a single biocompatible platform remains a challenge. We developed a device composed of an optical waveguide, six electrodes and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes while providing collocated neural recording and optical stimulation. The miniature ( < 200 μm) footprint and modest weight ( < 0.5 g) of these probes allowed for multiple implantations into the mouse brain, which enabled opto-electrophysiological investigation of projections from the basolateral amygdala to the medial prefrontal cortex and ventral hippocampus during behavioral experiments. Fabricated solely from polymers and polymer composites, these flexible probes minimized tissue response to achieve chronic multimodal interrogation of brain circuits with high fidelity.
DepartmentMassachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Materials Science and Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Research Laboratory of Electronics; McGovern Institute for Brain Research at MIT
Nature Publishing Group
Park, Seongjun et al. “One-Step Optogenetics with Multifunctional Flexible Polymer Fibers.” Nature Neuroscience 20, no. 4 (February 20, 2017): 612–619. © 2017 Nature America, Inc part of Springer Nature
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