Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array
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Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.
Date issued
2016-12Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MITJournal
Nature Biotechnology
Publisher
Nature Publishing Group
Citation
Zetsche, Bernd et al. “Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA Array.” Nature Biotechnology 35, 1 (December 2016): 31–34 © 2017 Nature America, Inc, part of Springer Nature
Version: Author's final manuscript
ISSN
1087-0156
1546-1696