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Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

dc.contributor.authorMohanraju, Prarthana
dc.contributor.authorKneppers, Jeroen
dc.contributor.authorDeGennaro, Ellen M
dc.contributor.authorWu, Wen Y
dc.contributor.authorScott, David A
dc.contributor.authorSeverinov, Konstantin
dc.contributor.authorvan der Oost, John
dc.contributor.authorZetsche, Bernd
dc.contributor.authorHeidenreich, Matthias
dc.contributor.authorFedorova, Iana
dc.contributor.authorWinblad, Nerges
dc.contributor.authorChoudhury, Sourav
dc.contributor.authorAbudayyeh, Omar Osama
dc.contributor.authorGootenberg, Jonathan S
dc.contributor.authorZhang, Feng
dc.date.accessioned2017-12-13T15:24:05Z
dc.date.available2017-12-13T15:24:05Z
dc.date.issued2016-12
dc.date.submitted2016-03
dc.identifier.issn1087-0156
dc.identifier.issn1546-1696
dc.identifier.urihttp://hdl.handle.net/1721.1/112728
dc.description.abstractTargeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.en_US
dc.description.sponsorshipNational Institute of Mental Health (U.S.) (Grant 5DP1-MH100706)en_US
dc.description.sponsorshipNational Institute of Mental Health (U.S.) (Grant 1R01-MH110049)en_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/NBT.3737en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleMultiplex gene editing by CRISPR–Cpf1 using a single crRNA arrayen_US
dc.typeArticleen_US
dc.identifier.citationZetsche, Bernd et al. “Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA Array.” Nature Biotechnology 35, 1 (December 2016): 31–34 © 2017 Nature America, Inc, part of Springer Natureen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.mitauthorZetsche, Bernd
dc.contributor.mitauthorHeidenreich, Matthias
dc.contributor.mitauthorFedorova, Iana
dc.contributor.mitauthorWinblad, Nerges
dc.contributor.mitauthorChoudhury, Sourav
dc.contributor.mitauthorAbudayyeh, Omar Osama
dc.contributor.mitauthorGootenberg, Jonathan S
dc.contributor.mitauthorZhang, Feng
dc.relation.journalNature Biotechnologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2017-12-12T18:45:35Z
dspace.orderedauthorsZetsche, Bernd; Heidenreich, Matthias; Mohanraju, Prarthana; Fedorova, Iana; Kneppers, Jeroen; DeGennaro, Ellen M; Winblad, Nerges; Choudhury, Sourav R; Abudayyeh, Omar O; Gootenberg, Jonathan S; Wu, Wen Y; Scott, David A; Severinov, Konstantin; van der Oost, John; Zhang, Fengen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7979-3220
dc.identifier.orcidhttps://orcid.org/0000-0003-2782-2509
mit.licenseOPEN_ACCESS_POLICYen_US


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