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dc.contributor.authorYu, Feiqiao Brian
dc.contributor.authorSchulz, Frederik
dc.contributor.authorWoyke, Tanja
dc.contributor.authorHorowitz, Mark A
dc.contributor.authorQuake, Stephen R
dc.contributor.authorBlainey, Paul C
dc.date.accessioned2018-02-14T14:08:58Z
dc.date.available2018-02-14T14:08:58Z
dc.date.issued2017-07
dc.date.submitted2017-03
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/1721.1/113647
dc.description.abstractMetagenomics and single-cell genomics have enabled genome discovery from unknown branches of life. However, extracting novel genomes from complex mixtures of metagenomic data can still be challenging and represents an ill-posed problem which is generally approached with ad hoc methods. Here we present a microfluidic-based mini-metagenomic method which offers a statistically rigorous approach to extract novel microbial genomes while preserving single-cell resolution. We used this approach to analyze two hot spring samples from Yellowstone National Park and extracted 29 new genomes, including three deeply branching lineages. The single-cell resolution enabled accurate quantification of genome function and abundance, down to 1% in relative abundance. Our analyses of genome level SNP distributions also revealed low to moderate environmental selection. The scale, resolution, and statistical power of microfluidic-based mini-metagenomics make it a powerful tool to dissect the genomic structure of microbial communities while effectively preserving the fundamental unit of biology, the single cell.en_US
dc.publishereLife Sciences Publications, Ltden_US
dc.relation.isversionofhttp://dx.doi.org/10.7554/ELIFE.26580en_US
dc.rightsCreative Commons Attribution 4.0 International Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceeLifeen_US
dc.titleMicrofluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samplesen_US
dc.typeArticleen_US
dc.identifier.citationYu, Feiqiao Brian et al. “Microfluidic-Based Mini-Metagenomics Enables Discovery of Novel Microbial Lineages from Complex Environmental Samples.” eLife 6 (July 2017): e26580 © 2017 Yu et alen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.mitauthorBlainey, Paul C
dc.relation.journaleLifeen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-02-14T13:59:03Z
dspace.orderedauthorsYu, Feiqiao Brian; Blainey, Paul C; Schulz, Frederik; Woyke, Tanja; Horowitz, Mark A; Quake, Stephen Ren_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7014-3830
mit.licensePUBLISHER_CCen_US


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