PD-L1 is an activation-independent marker of brown adipocytes
Author(s)Ingram, Jessica R.; Dougan, Michael; Rashidian, Mohammad; Knoll, Marko; Keliher, Edmund J.; Garrett, Sarah; Garforth, Scott; Blomberg, Olga S.; Espinosa, Camilo; Bhan, Atul; Almo, Steven C.; Weissleder, Ralph; Dougan, Stephanie K.; Lodish, Harvey F; Ploegh, Hidde; ... Show more Show less
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Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.
DepartmentMassachusetts Institute of Technology. Department of Biology
Ingram, Jessica R. et al. “PD-L1 Is an Activation-Independent Marker of Brown Adipocytes.” Nature Communications 8, 1 (September 2017): 647 © 2017 The Author(s)
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