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dc.contributor.authorIngram, Jessica R.
dc.contributor.authorDougan, Michael
dc.contributor.authorRashidian, Mohammad
dc.contributor.authorKnoll, Marko
dc.contributor.authorKeliher, Edmund J.
dc.contributor.authorGarrett, Sarah
dc.contributor.authorGarforth, Scott
dc.contributor.authorBlomberg, Olga S.
dc.contributor.authorEspinosa, Camilo
dc.contributor.authorBhan, Atul
dc.contributor.authorAlmo, Steven C.
dc.contributor.authorWeissleder, Ralph
dc.contributor.authorDougan, Stephanie K.
dc.contributor.authorLodish, Harvey F
dc.contributor.authorPloegh, Hidde
dc.date.accessioned2018-05-02T20:48:40Z
dc.date.available2018-05-02T20:48:40Z
dc.date.issued2017-09
dc.date.submitted2017-02
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/1721.1/115203
dc.description.abstractProgrammed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 2R01 DK047618-28)en_US
dc.description.sponsorshipNational Institute of Diabetes and Digestive and Kidney Diseases (U.S.) (Grant 2R01 DK047618-28)en_US
dc.publisherSpringer Natureen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/S41467-017-00799-8en_US
dc.rightsAttribution 4.0 International (CC BY 4.0)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Communicationsen_US
dc.titlePD-L1 is an activation-independent marker of brown adipocytesen_US
dc.typeArticleen_US
dc.identifier.citationIngram, Jessica R. et al. “PD-L1 Is an Activation-Independent Marker of Brown Adipocytes.” Nature Communications 8, 1 (September 2017): 647 © 2017 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorLodish, Harvey F
dc.contributor.mitauthorPloegh, Hidde
dc.relation.journalNature Communicationsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-04-27T14:53:02Z
dspace.orderedauthorsIngram, Jessica R.; Dougan, Michael; Rashidian, Mohammad; Knoll, Marko; Keliher, Edmund J.; Garrett, Sarah; Garforth, Scott; Blomberg, Olga S.; Espinosa, Camilo; Bhan, Atul; Almo, Steven C.; Weissleder, Ralph; Lodish, Harvey; Dougan, Stephanie K.; Ploegh, Hidde L.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7029-7415
dc.identifier.orcidhttps://orcid.org/0000-0002-1090-6071
mit.licensePUBLISHER_CCen_US


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