dc.contributor.author | Ingram, Jessica R. | |
dc.contributor.author | Dougan, Michael | |
dc.contributor.author | Rashidian, Mohammad | |
dc.contributor.author | Knoll, Marko | |
dc.contributor.author | Keliher, Edmund J. | |
dc.contributor.author | Garrett, Sarah | |
dc.contributor.author | Garforth, Scott | |
dc.contributor.author | Blomberg, Olga S. | |
dc.contributor.author | Espinosa, Camilo | |
dc.contributor.author | Bhan, Atul | |
dc.contributor.author | Almo, Steven C. | |
dc.contributor.author | Weissleder, Ralph | |
dc.contributor.author | Dougan, Stephanie K. | |
dc.contributor.author | Lodish, Harvey F | |
dc.contributor.author | Ploegh, Hidde | |
dc.date.accessioned | 2018-05-02T20:48:40Z | |
dc.date.available | 2018-05-02T20:48:40Z | |
dc.date.issued | 2017-09 | |
dc.date.submitted | 2017-02 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/115203 | |
dc.description.abstract | Programmed death ligand 1 (PD-L1) is expressed on a number of immune and cancer cells, where it can downregulate antitumor immune responses. Its expression has been linked to metabolic changes in these cells. Here we develop a radiolabeled camelid single-domain antibody (anti-PD-L1 VHH) to track PD-L1 expression by immuno-positron emission tomography (PET). PET-CT imaging shows a robust and specific PD-L1 signal in brown adipose tissue (BAT). We confirm expression of PD-L1 on brown adipocytes and demonstrate that signal intensity does not change in response to cold exposure or β-adrenergic activation. This is the first robust method of visualizing murine brown fat independent of its activation state. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant 2R01 DK047618-28) | en_US |
dc.description.sponsorship | National Institute of Diabetes and Digestive and Kidney Diseases (U.S.) (Grant 2R01 DK047618-28) | en_US |
dc.publisher | Springer Nature | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/S41467-017-00799-8 | en_US |
dc.rights | Attribution 4.0 International (CC BY 4.0) | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nature Communications | en_US |
dc.title | PD-L1 is an activation-independent marker of brown adipocytes | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Ingram, Jessica R. et al. “PD-L1 Is an Activation-Independent Marker of Brown Adipocytes.” Nature Communications 8, 1 (September 2017): 647 © 2017 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Lodish, Harvey F | |
dc.contributor.mitauthor | Ploegh, Hidde | |
dc.relation.journal | Nature Communications | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2018-04-27T14:53:02Z | |
dspace.orderedauthors | Ingram, Jessica R.; Dougan, Michael; Rashidian, Mohammad; Knoll, Marko; Keliher, Edmund J.; Garrett, Sarah; Garforth, Scott; Blomberg, Olga S.; Espinosa, Camilo; Bhan, Atul; Almo, Steven C.; Weissleder, Ralph; Lodish, Harvey; Dougan, Stephanie K.; Ploegh, Hidde L. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7029-7415 | |
dc.identifier.orcid | https://orcid.org/0000-0002-1090-6071 | |
mit.license | PUBLISHER_CC | en_US |