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Ubiquitin-Dependent Control of Class II MHC Localization Is Dispensable for Antigen Presentation and Antibody Production

Author(s)
McGehee, Annette M.; Strijbis, Karin; Guillen, Eduardo; Eng, Thomas; Kirak, Oktay; Ploegh, Hidde; ... Show more Show less
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Abstract
Controlled localization of class II MHC molecules is essential for proper class II MHC-restricted antigen presentation and the subsequent initiation of an adaptive immune response. Ubiquitination of class II MHC molecules on cytosolic lysine (K225) of the β-chain has been shown to affect localization of the complex. We generated mice in which the endogenous β-chain locus is replaced with a GFP tagged mutant version that lacks the cytosolic lysine residue (I-A-β-K225R-EGFP). These mice have elevated levels of class II MHC as compared to I-A-β-EGFP mice, and immature bone marrow-derived dendritic cells show redistribution of class II MHC to the cell surface. Nonetheless, in these same cells efficiency of antigen presentation is unaffected in I-A-β-K225R-EGFP mice, as assayed for presentation of ovalbumin to appropriately specific T cells. The I-A-β-K225R-EGFP animals have normal CD4 T cell populations and are capable of generating antigen-specific antibody in response to model antigens and viral infection. We therefore conclude that in our experimental system modulation of trafficking by ubiquitination of residue K225 of the β-chain is not essential for the function of class II MHC products in antigen presentation or antibody production.
Date issued
2011-04
URI
http://hdl.handle.net/1721.1/116199
Department
Massachusetts Institute of Technology. Department of Biology
Journal
PLoS ONE
Publisher
Public Library of Science (PLoS)
Citation
McGehee, Annette M. et al. “Ubiquitin-Dependent Control of Class II MHC Localization Is Dispensable for Antigen Presentation and Antibody Production.” Edited by Marcus R. Clark. PLoS ONE 6, 4 (April 2011): e18817 © 2011 McGehee et al
Version: Final published version
ISSN
1932-6203

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