dc.contributor.author | McGehee, Annette M. | |
dc.contributor.author | Strijbis, Karin | |
dc.contributor.author | Guillen, Eduardo | |
dc.contributor.author | Eng, Thomas | |
dc.contributor.author | Kirak, Oktay | |
dc.contributor.author | Ploegh, Hidde | |
dc.date.accessioned | 2018-06-11T15:01:20Z | |
dc.date.available | 2018-06-11T15:01:20Z | |
dc.date.issued | 2011-04 | |
dc.date.submitted | 2010-12 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/116199 | |
dc.description.abstract | Controlled localization of class II MHC molecules is essential for proper class II MHC-restricted antigen presentation and the subsequent initiation of an adaptive immune response. Ubiquitination of class II MHC molecules on cytosolic lysine (K225) of the β-chain has been shown to affect localization of the complex. We generated mice in which the endogenous β-chain locus is replaced with a GFP tagged mutant version that lacks the cytosolic lysine residue (I-A-β-K225R-EGFP). These mice have elevated levels of class II MHC as compared to I-A-β-EGFP mice, and immature bone marrow-derived dendritic cells show redistribution of class II MHC to the cell surface. Nonetheless, in these same cells efficiency of antigen presentation is unaffected in I-A-β-K225R-EGFP mice, as assayed for presentation of ovalbumin to appropriately specific T cells. The I-A-β-K225R-EGFP animals have normal CD4 T cell populations and are capable of generating antigen-specific antibody in response to model antigens and viral infection. We therefore conclude that in our experimental system modulation of trafficking by ubiquitination of residue K225 of the β-chain is not essential for the function of class II MHC products in antigen presentation or antibody production. | en_US |
dc.publisher | Public Library of Science (PLoS) | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1371/journal.pone.0018817 | en_US |
dc.rights | Attribution 4.0 International (CC BY 4.0) | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | PLoS | en_US |
dc.title | Ubiquitin-Dependent Control of Class II MHC Localization Is Dispensable for Antigen Presentation and Antibody Production | en_US |
dc.type | Article | en_US |
dc.identifier.citation | McGehee, Annette M. et al. “Ubiquitin-Dependent Control of Class II MHC Localization Is Dispensable for Antigen Presentation and Antibody Production.” Edited by Marcus R. Clark. PLoS ONE 6, 4 (April 2011): e18817 © 2011 McGehee et al | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Ploegh, Hidde | |
dc.relation.journal | PLoS ONE | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2018-06-08T18:35:27Z | |
dspace.orderedauthors | McGehee, Annette M.; Strijbis, Karin; Guillen, Eduardo; Eng, Thomas; Kirak, Oktay; Ploegh, Hidde L. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-1090-6071 | |
mit.license | PUBLISHER_CC | en_US |