Whole-Mount X-Gal Staining of Mouse Tissues
Author(s)Gierut, J. J.; Haigis, K. M.; Jacks, Tyler E.
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Although the development of improved mouse models, including conditional deletions, marks an exciting time in mouse genetics, it is important to characterize and validate these models. Cre reporter strains allow researchers to assess the recombinase expression profile and function in individual Cre mouse lines. These strains are engineered to express a reporter gene (usually LacZ) following the removal of a floxed STOP cassette, thus marking cell lineages that can be targeted with a given Cre line. This protocol provides a detailed method for the histochemical detection of β-galactosidase activity in Cre mouse strains.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT
Cold Spring Harbor Protocols
Cold Spring Harbor Laboratory Press
Gierut, J. J. et al. “Whole-Mount X-Gal Staining of Mouse Tissues.” Cold Spring Harbor Protocols 2014, 4 (April 2014): 417-419 © 2014 Cold Spring Harbor Laboratory Press
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