Whole-Mount X-Gal Staining of Mouse Tissues
Author(s)
Gierut, J. J.; Haigis, K. M.; Jacks, Tyler E.
Downloadnihms621453.pdf (61.84Kb)
OPEN_ACCESS_POLICY
Open Access Policy
Creative Commons Attribution-Noncommercial-Share Alike
Terms of use
Metadata
Show full item recordAbstract
Although the development of improved mouse models, including conditional deletions, marks an exciting time in mouse genetics, it is important to characterize and validate these models. Cre reporter strains allow researchers to assess the recombinase expression profile and function in individual Cre mouse lines. These strains are engineered to express a reporter gene (usually LacZ) following the removal of a floxed STOP cassette, thus marking cell lineages that can be targeted with a given Cre line. This protocol provides a detailed method for the histochemical detection of β-galactosidase activity in Cre mouse strains.
Date issued
2014-04Department
Koch Institute for Integrative Cancer Research at MITJournal
Cold Spring Harbor Protocols
Publisher
Cold Spring Harbor Laboratory Press
Citation
Gierut, J. J. et al. “Whole-Mount X-Gal Staining of Mouse Tissues.” Cold Spring Harbor Protocols 2014, 4 (April 2014): 417-419 © 2014 Cold Spring Harbor Laboratory Press
Version: Author's final manuscript
ISSN
1559-6095
1940-3402