MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Multiparameter cell-tracking intrinsic cytometry for single-cell characterization

Author(s)
Apichitsopa, Nicha; Jaffe, Alexander S.; Voldman, Joel
Thumbnail
Downloadc8lc00240a.pdf (3.918Mb)
PUBLISHER_CC

Publisher with Creative Commons License

Creative Commons Attribution

Terms of use
Creative Commons Attribution-NonCommercial 3.0 Unported https://creativecommons.org/licenses/by-nc/3.0/
Metadata
Show full item record
Abstract
An abundance of label-free microfluidic techniques for measuring cell intrinsic markers exists, yet these techniques are seldom combined because of integration complexity such as restricted physical space and incompatible modes of operation. We introduce a multiparameter intrinsic cytometry approach for the characterization of single cells that combines ≥2 label-free measurement techniques onto the same platform and uses cell tracking to associate the measured properties to cells. Our proof-of-concept implementation can measure up to five intrinsic properties including size, deformability, and polarizability at three frequencies. Each measurement module along with the integrated platform were validated and evaluated in the context of chemically induced changes in the actin cytoskeleton of cells. viSNE and machine learning classification were used to determine the orthogonality between and the contribution of the measured intrinsic markers for cell classification.
Date issued
2018-04
URI
http://hdl.handle.net/1721.1/116896
Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
Citation
Apichitsopa, N., A. Jaffe, and J. Voldman. “Multiparameter Cell-Tracking Intrinsic Cytometry for Single-Cell Characterization.” Lab on a Chip 18, 10 (2018): 1430–1439 © 2018 Royal Society of Chemistry
Version: Final published version
ISSN
1473-0197
1473-0189

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.