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dc.contributor.authorKearney, Sean M
dc.contributor.authorGibbons, Sean Michael
dc.contributor.authorErdman, Susan E
dc.contributor.authorAlm, Eric J
dc.date.accessioned2018-08-28T12:33:36Z
dc.date.available2018-08-28T12:33:36Z
dc.date.issued2018-08
dc.date.submitted2018-06
dc.identifier.issn22111247
dc.identifier.urihttp://hdl.handle.net/1721.1/117571
dc.description.abstractInterest in manipulating the gut microbiota to treat disease has led to a need for understanding how organisms can establish themselves when introduced into a host with an intact microbial community. While probiotic or prebiotic approaches typically lead to a transient pulse in abundance of an organism, persistent establishment of an introduced species may require alternative strategies. Here, we introduce the concept of orthogonal niche engineering in the gut, where we include a resource typically absent from the diet, seaweed, to establish a customized niche for an introduced organism. We show that in the short term, co-introduction of this resource at 1% in the diet along with an organism with exclusive access to this resource, B. plebeius DSM 17135, enables it to colonize at a median abundance of 1%, frequently increasing in abundance to 10 or more percent. We construct a mathematical model of the system to infer that B. plebeius competitively acquires endogenous resources. We provide evidence that it competes with native commensals to achieve its observed abundance. We observe a diet-dependent loss in seaweed responsiveness of B. plebeius in the long term and show the potential for IgA-mediated control of putative invaders by the immune system. These results point to the potential for diet-based intervention as a means to introduce target organisms, but also indicate potential modes for failure of this strategy in the long term.en_US
dc.description.sponsorshipBroad Institute of MIT and Harvard (BN10 Training Grants)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Graduate Research Fellowship (DGE-1122374))en_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.celrep.2018.07.032en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourceElsevieren_US
dc.titleOrthogonal Dietary Niche Enables Reversible Engraftment of a Gut Bacterial Commensalen_US
dc.typeArticleen_US
dc.identifier.citationKearney, Sean M., Sean M. Gibbons, Susan E. Erdman, and Eric J. Alm. “Orthogonal Dietary Niche Enables Reversible Engraftment of a Gut Bacterial Commensal.” Cell Reports 24, no. 7 (August 2018): 1842–1851.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Division of Comparative Medicineen_US
dc.contributor.mitauthorKearney, Sean M
dc.contributor.mitauthorGibbons, Sean Michael
dc.contributor.mitauthorErdman, Susan E
dc.contributor.mitauthorAlm, Eric J
dc.relation.journalCell Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2018-08-23T14:45:24Z
dspace.orderedauthorsKearney, Sean M.; Gibbons, Sean M.; Erdman, Susan E.; Alm, Eric J.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-8033-8380
dc.identifier.orcidhttps://orcid.org/0000-0001-8294-9364
mit.licensePUBLISHER_CCen_US


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