3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade
Name
c8lc00322j.pdf
Size
5.01 MB
Format
Adobe PDF
Checksum (MD5)
46dcde9309167f0a35d02b80db8093ce
Author(s)
Portell, Andrew
Larios, Dalia
Piel, Brandon P.
Mathur, Natasha
Zhou, Chensheng
Coakley, Raven Vlahos
Bartels, Alan
Bowden, Michaela
Herbert, Zach
Gilhooley, Sean
Date Issued
September 2018
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
Citation
Aref, Amir R., Marco Campisi, Elena Ivanova, Andrew Portell, Dalia Larios, Brandon P. Piel, Natasha Mathur, et al. “3D Microfluidic Ex Vivo Culture of Organotypic Tumor Spheroids to Model Immune Checkpoint Blockade.” Lab on a Chip (2018).
Version
Final published version
Abstract
Microfluidic culture has the potential to revolutionize cancer diagnosis and therapy. Indeed, several micro- devices are being developed specifically for clinical use to test novel cancer therapeutics. To be effective, these platforms need to replicate the continuous interactions that exist between tumor cells and non- tumor cell elements of the tumor microenvironment through direct cell – cell or cell – matrix contact or by the secretion of signaling factors such as cytokines, chemokines and growth factors. Given the challenges of personalized or precision cancer therapy, especially with the advent of novel immunotherapies, a critical need exists for more sophisticated ex vivo diagnostic systems that recapitulate patient-specific tumor biol- ogy with the potential to predict response to immune-based therapies in real-time. Here, we present de- tails of a method to screen for the response of patient tumors to immune checkpoint blockade therapy, first reported in Jenkins et al. Cancer Discovery, 2018, 8,196 – 215, with updated evaluation of murine- and patient-derived organotypic tumor spheroids (MDOTS/PDOTS), including evaluation of the requirement for 3D microfluidic culture in MDOTS, demonstration of immune-checkpoint sensitivity of PDOTS, and ex- panded evaluation of tumor – immune interactions using RNA-sequencing to infer changes in the tumor – immune microenvironment. We also examine some potential improvements to current systems and dis- cuss the challenges in translating such diagnostic assays to the clinic.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial 3.0 Unported
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1039/C8LC00322J
