Image formation of volume holographic microscopy using point spread functions
Author(s)
Kou, Shan Shan
Sheppard, Colin J. R.
Luo, Yuan
Oh, Se Baek
Lee, Justin Wu
Barbastathis, George
Date Issued
March 2010
Journal
Proceedings Volume 7622, Medical Imaging 2010: Physics of Medical Imaging
Publisher
SPIE
Citation
Luo, Yuan, et al. "Image Formation of Volume Holographic Microscopy Using Point Spread Functions." Proceedings Volume 7622, Medical Imaging 2010: Physics of Medical Imaging, 15-18 March, 2010, San Diego, California, 2010, SPIE, p. 76224E. © 2010 Society of Photo-Optical Instrumentation Engineers (SPIE)
Version
Final published version
Abstract
We present a theoretical formulation to quantify the imaging properties of volume holographic microscopy (VHM). Volume holograms are formed by exposure of a photosensitive recording material to the interference of two mutually coherent optical fields. Recently, it has been shown that a volume holographic pupil has spatial and spectral sectioning capability for fluorescent samples. Here, we analyze the point spread function (PSF) to assess the imaging behavior of the VHM with a point source and detector. The coherent PSF of the VHM is derived, and the results are compared with those from conventional microscopy, and confocal microscopy with point and slit apertures. According to our analysis, the PSF of the VHM can be controlled in the lateral direction by adjusting the parameters of the VH. Compared with confocal microscopes, the performance of the VHM is comparable or even potentially better, and the VHM is also able to achieve real-time and three-dimensional (3D) imaging due to its multiplexing ability. © 2010 SPIE.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1117/12.844310
