Expansion microscopy: enabling single cell analysis in intact biological systems
Author(s)
Alon, Shahar; Huynh, Grace H.; Boyden, Edward S.
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There is a need for single cell analysis methods that enable the identification and localization of different kinds of biomolecules throughout cells and intact tissues, thereby allowing characterization and classification of individual cells and their relationships to each other within intact systems. Expansion microscopy (ExM) is a technology that physically magnifies tissues in an isotropic way, thereby achieving super-resolution microscopy on diffraction-limited microscopes, enabling rapid image acquisition and large field of view. As a result, ExM is well-positioned to integrate molecular content and cellular morphology, with the spatial precision sufficient to resolve individual biological building blocks, and the scale and accessibility required to deploy over extended 3-D objects like tissues and organs. ©2019
Date issued
2019-04Department
Massachusetts Institute of Technology. Media Laboratory; McGovern Institute for Brain Research at MIT; Massachusetts Institute of Technology. Department of Biological Engineering; Koch Institute for Integrative Cancer Research at MITJournal
FEBS journal
Publisher
Wiley
Citation
Alon, Shahar, Grace H. Huynh, and Edward S. Boyden, "Expansion microscopy: enabling single cell analysis in intact biological systems." FEBS journal 286, 8 (April 2019): p. 1482-94 doi 10.1111/febs.14597 2019 ©2019 Author(s)
Version: Author's final manuscript
ISSN
1742-4658