dc.contributor.author | Christ, Bastien | |
dc.contributor.author | Xu, Chengchao | |
dc.contributor.author | Xu, Menglong | |
dc.contributor.author | Li, Fu-Shuang | |
dc.contributor.author | Mitchell, Andrew J. | |
dc.contributor.author | Weng, Jing-Ke | |
dc.date.accessioned | 2020-04-17T12:01:10Z | |
dc.date.available | 2020-04-17T12:01:10Z | |
dc.date.issued | 2019-07-19 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/124710 | |
dc.description.abstract | Diosgenin is a spiroketal steroidal natural product extracted from plants and used as the single most important precursor for the world steroid hormone industry. The sporadic occurrences of diosgenin in distantly related plants imply possible independent biosynthetic origins. The characteristic 5,6-spiroketal moiety in diosgenin is reminiscent of the spiroketal moiety present in anthelmintic avermectins isolated from actinomycete bacteria. How plants gained the ability to biosynthesize spiroketal natural products is unknown. Here, we report the diosgenin-biosynthetic pathways in himalayan paris (Paris polyphylla), a monocot medicinal plant with hemostatic and antibacterial properties, and fenugreek (Trigonella foenum–graecum), an eudicot culinary herb plant commonly used as a galactagogue. Both plants have independently recruited pairs of cytochromes P450 that catalyze oxidative 5,6-spiroketalization of cholesterol to produce diosgenin, with evolutionary progenitors traced to conserved phytohormone metabolism. This study paves the way for engineering the production of diosgenin and derived analogs in heterologous hosts. | en_US |
dc.description.sponsorship | Pew Scholars Program in the Biomedical Sciences (Grant (27345) | en_US |
dc.description.sponsorship | Searle Scholars Program (Grant 15-SSP-162) | en_US |
dc.language.iso | en | |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.relation.isversionof | 10.1038/s41467-019-11286-7 | en_US |
dc.rights | Creative Commons Attribution 4.0 International license | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nature | en_US |
dc.subject | General Biochemistry, Genetics and Molecular Biology | en_US |
dc.subject | General Physics and Astronomy | en_US |
dc.subject | General Chemistry | en_US |
dc.title | Repeated evolution of cytochrome P450-mediated spiroketal steroid biosynthesis in plants | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Christ, Bastien et al. "Repeated evolution of cytochrome P450-mediated spiroketal steroid biosynthesis in plants." Nature communications 10 (2019): 1038 © 2019 The Author(s) | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.relation.journal | Nature communications | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2020-02-03T20:16:04Z | |
dspace.date.submission | 2020-02-03T20:16:06Z | |
mit.journal.volume | 10 | en_US |
mit.journal.issue | 1 | en_US |
mit.license | PUBLISHER_CC | |
mit.metadata.status | Complete | |