NUFIP1 is a ribosome receptor for starvation-induced ribophagy
Author(s)Wyant, Gregory A.; Abu-Remaileh, Monther; Frenkel, Evgeni M.; Laqtom, Nouf N.; Dharamdasani, Vimisha; Lewis, Caroline A.; Chan, Sze Ham; Sabatini, David M.; ... Show more Show less
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The lysosome degrades and recycles macromolecules, signals to the master growth regulator mTORC1 [mechanistic target of rapamycin (mTOR) complex 1], and is associated with human disease.We performed quantitative proteomic analyses of rapidly isolated lysosomes and found that nutrient levels and mTOR dynamically modulate the lysosomal proteome. Upon mTORC1 inhibition, NUFIP1 (nuclear fragile Xmental retardation-interacting protein 1) redistributes from the nucleus to autophagosomes and lysosomes. Upon these conditions, NUFIP1 interacts with ribosomes and delivers them to autophagosomes by directly binding to microtubule-associated proteins 1A/1B light chain 3B (LC3B).The starvation-induced degradation of ribosomes via autophagy (ribophagy) depends on the capacity of NUFIP1 to bind LC3B and promotes cell survival.We propose that NUFIP1 is a receptor for the selective autophagy of ribosomes.
DepartmentWhitehead Institute for Biomedical Research; Massachusetts Institute of Technology. Department of Biology; David H. Koch Institute for Integrative Cancer Research at MIT
American Association for the Advancement of Science (AAAS)
Wyant, Gregory A. et al. "NUFIP1 is a ribosome receptor for starvation-induced ribophagy." Science 360 (2018): 751-758 © 2018 The Author(s)
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