Show simple item record

Diversity and evolution of class 2 CRISPR–Cas systems

dc.contributor.authorShmakov, Sergey
dc.contributor.authorSmargon, Aaron Andrew
dc.contributor.authorScott, David (David Arthur)
dc.contributor.authorCox, David D.
dc.contributor.authorPyzocha, Neena
dc.contributor.authorYan, Winston Xia
dc.contributor.authorAbudayyeh, Omar O.
dc.contributor.authorGootenberg, Jonathan S
dc.contributor.authorMakarova, Kira S.
dc.contributor.authorWolf, Yuri I.
dc.contributor.authorSeverinov, Konstantin
dc.contributor.authorZhang, Feng
dc.contributor.authorKoonin, Eugene V.
dc.date.accessioned2020-05-20T19:02:48Z
dc.date.available2020-05-20T19:02:48Z
dc.date.issued2017-01
dc.identifier.issn1740-1526
dc.identifier.issn1740-1534
dc.identifier.urihttps://hdl.handle.net/1721.1/125361
dc.description.abstractClass 2 CRISPR-Cas systems are characterized by effector modules that consist of a single multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the discovery of novel class 2 variants and used it to identify six new CRISPR-Cas subtypes. The diverse properties of these new systems provide potential for the development of versatile tools for genome editing and regulation. In this Analysis article, we present a comprehensive census of class 2 types and class 2 subtypes in complete and draft bacterial and archaeal genomes, outline evolutionary scenarios for the independent origin of different class 2 CRISPR-Cas systems from mobile genetic elements, and propose an amended classification and nomenclature of CRISPR-Cas. Keywords: Bacterial evolution; Bacterial genetics; CRISPR-Cas systemsen_US
dc.description.sponsorshipUS National Institute of Mental Health (Grant 5DP1-MH100706)en_US
dc.description.sponsorshipUS National Institute of Mental Health (Grant 1R01-MH110049)en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nrmicro.2016.184en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleDiversity and evolution of class 2 CRISPR–Cas systemsen_US
dc.typeArticleen_US
dc.identifier.citationShmakov, Sergey et al. "Diversity and evolution of class 2 CRISPR–Cas systems." Nature Reviews Microbiology 15, 3 (March 2017): 169–182en_US
dc.contributor.departmentBroad Institute of MIT and Harvarden_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.relation.journalNature Reviews Microbiologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-10-08T12:18:08Z
dspace.date.submission2019-10-08T12:18:11Z
mit.journal.volume15en_US
mit.journal.issue3en_US
mit.metadata.statusComplete


Files in this item

Thumbnail
Name:
nihms945745.pdf
Size:
1.966Mb
Format:
PDF
Description:
Accepted version
View/Open

This item appears in the following Collection(s)

Show simple item record