One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

Author(s)
Jacobsen, Johanne T.; Mesin, Luka; Markoulaki, Styliani; Schiepers, Ariën; Cavazzoni, Cecília B.; ... Show more
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Abstract
We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.
Date issued
2018-09
URI
https://hdl.handle.net/1721.1/125966
Department
Massachusetts Institute of Technology. Department of Biology
Journal
Journal of Experimental Medicine
Publisher
Rockefeller University Press
Citation
Jacobsen, Johanne T. et al. "One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation." Journal of Experimental Medicine 215, 10 (September 2018): 2686–2695 © 2018 Jacobsen et al
Version: Final published version
ISSN
0022-1007
1540-9538
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