| dc.contributor.author | Jacobsen, Johanne T. | |
| dc.contributor.author | Mesin, Luka | |
| dc.contributor.author | Markoulaki, Styliani | |
| dc.contributor.author | Schiepers, Ariën | |
| dc.contributor.author | Cavazzoni, Cecília B. | |
| dc.contributor.author | Bousbaine, Djenet | |
| dc.contributor.author | Jaenisch, Rudolf | |
| dc.contributor.author | Victora, Gabriel D. | |
| dc.date.accessioned | 2020-06-23T21:22:27Z | |
| dc.date.available | 2020-06-23T21:22:27Z | |
| dc.date.issued | 2018-09 | |
| dc.date.submitted | 2018-07 | |
| dc.identifier.issn | 0022-1007 | |
| dc.identifier.issn | 1540-9538 | |
| dc.identifier.uri | https://hdl.handle.net/1721.1/125966 | |
| dc.description.abstract | We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation. | en_US |
| dc.description.sponsorship | National Institute of Allergy and Infectious Diseases (Grant R01AI119006) | en_US |
| dc.description.sponsorship | National Institute of Allergy and Infectious Diseases (Grant R21AI138020) | en_US |
| dc.language.iso | en | |
| dc.publisher | Rockefeller University Press | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1084/jem.20172064 | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
| dc.source | Rockefeller University Press | en_US |
| dc.title | One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Jacobsen, Johanne T. et al. "One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation." Journal of Experimental Medicine 215, 10 (September 2018): 2686–2695 © 2018 Jacobsen et al | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
| dc.relation.journal | Journal of Experimental Medicine | en_US |
| dc.eprint.version | Final published version | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dc.date.updated | 2019-12-10T19:38:54Z | |
| dspace.date.submission | 2019-12-10T19:38:55Z | |
| mit.journal.volume | 215 | en_US |
| mit.journal.issue | 10 | en_US |
| mit.metadata.status | Complete | |
