Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling

Author(s)
Myers, Samuel A.; Wright, Jason; Peckner, Ryan; Kalish, Brian T.; Zhang, Feng; ... Show more
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Abstract
Regulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.
Date issued
2018-05
URI
https://hdl.handle.net/1721.1/126374
Department
Broad Institute of MIT and Harvard; Massachusetts Institute of Technology. Department of Biology
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Meyers, Samuel A. et al. "Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling." Nature Methods 15, 6 (May 2018): 437–439 © 2018 The Author(s)
Version: Author's final manuscript
ISSN
1548-7091
1548-7105
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