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Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling

dc.contributor.authorMyers, Samuel A.
dc.contributor.authorWright, Jason
dc.contributor.authorPeckner, Ryan
dc.contributor.authorKalish, Brian T.
dc.contributor.authorZhang, Feng
dc.contributor.authorCarr, Steven A
dc.date.accessioned2020-07-24T17:47:46Z
dc.date.available2020-07-24T17:47:46Z
dc.date.issued2018-05
dc.date.submitted2017-12
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttps://hdl.handle.net/1721.1/126374
dc.description.abstractRegulation of gene expression is primarily controlled by changes in the proteins that occupy genes' regulatory elements. We developed genomic locus proteomics (GLoPro), in which we combine CRISPR-based genome targeting, proximity labeling, and quantitative proteomics to discover proteins associated with a specific genomic locus in native cellular contexts.en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/s41592-018-0007-1en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleDiscovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labelingen_US
dc.typeArticleen_US
dc.identifier.citationMeyers, Samuel A. et al. "Discovery of proteins associated with a predefined genomic locus via dCas9–APEX-mediated proximity labeling." Nature Methods 15, 6 (May 2018): 437–439 © 2018 The Author(s)en_US
dc.contributor.departmentBroad Institute of MIT and Harvarden_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-10-08T13:08:42Z
dspace.date.submission2019-10-08T13:08:43Z
mit.journal.volume15en_US
mit.journal.issue6en_US
mit.metadata.statusComplete


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