Noninvasive monitoring of single-cell mechanics by acoustic scattering
Author(s)Kang, Joon Ho; Miettinen, Teemu P; Chen, Lynna; Olcum, Selim A.; Katsikis, Georgios; Doyle, Patrick S; Manalis, Scott R; ... Show more Show less
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The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that acoustic scattering can be used to noninvasively probe subtle and transient dynamics.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Physics; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
Springer Science and Business Media LLC
Kang, Joon Ho et al. "Noninvasive monitoring of single-cell mechanics by acoustic scattering." Nature Methods 16, 3 (February 2019): 263–269 © 2019 The Author(s)
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