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dc.contributor.authorKang, Joon Ho
dc.contributor.authorMiettinen, Teemu P
dc.contributor.authorChen, Lynna
dc.contributor.authorOlcum, Selim A.
dc.contributor.authorKatsikis, Georgios
dc.contributor.authorDoyle, Patrick S
dc.contributor.authorManalis, Scott R
dc.date.accessioned2020-12-15T22:56:53Z
dc.date.available2020-12-15T22:56:53Z
dc.date.issued2019-02
dc.date.submitted2018-09
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttps://hdl.handle.net/1721.1/128840
dc.description.abstractThe monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that acoustic scattering can be used to noninvasively probe subtle and transient dynamics.en_US
dc.language.isoen
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/s41592-019-0326-xen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleNoninvasive monitoring of single-cell mechanics by acoustic scatteringen_US
dc.typeArticleen_US
dc.identifier.citationKang, Joon Ho et al. "Noninvasive monitoring of single-cell mechanics by acoustic scattering." Nature Methods 16, 3 (February 2019): 263–269 © 2019 The Author(s)en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Physicsen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2019-08-16T17:02:07Z
dspace.date.submission2019-08-16T17:02:09Z
mit.journal.volume16en_US
mit.journal.issue3en_US
mit.metadata.statusComplete


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