dc.contributor.author | Kang, Joon Ho | |
dc.contributor.author | Miettinen, Teemu P | |
dc.contributor.author | Chen, Lynna | |
dc.contributor.author | Olcum, Selim A. | |
dc.contributor.author | Katsikis, Georgios | |
dc.contributor.author | Doyle, Patrick S | |
dc.contributor.author | Manalis, Scott R | |
dc.date.accessioned | 2020-12-15T22:56:53Z | |
dc.date.available | 2020-12-15T22:56:53Z | |
dc.date.issued | 2019-02 | |
dc.date.submitted | 2018-09 | |
dc.identifier.issn | 1548-7091 | |
dc.identifier.issn | 1548-7105 | |
dc.identifier.uri | https://hdl.handle.net/1721.1/128840 | |
dc.description.abstract | The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that acoustic scattering can be used to noninvasively probe subtle and transient dynamics. | en_US |
dc.language.iso | en | |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/s41592-019-0326-x | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | PMC | en_US |
dc.title | Noninvasive monitoring of single-cell mechanics by acoustic scattering | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Kang, Joon Ho et al. "Noninvasive monitoring of single-cell mechanics by acoustic scattering." Nature Methods 16, 3 (February 2019): 263–269 © 2019 The Author(s) | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Physics | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Mechanical Engineering | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.relation.journal | Nature Methods | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2019-08-16T17:02:07Z | |
dspace.date.submission | 2019-08-16T17:02:09Z | |
mit.journal.volume | 16 | en_US |
mit.journal.issue | 3 | en_US |
mit.metadata.status | Complete | |