Multi-Omics Characterization of Inflammatory Bowel Disease-Induced Hyperplasia/Dysplasia in the Rag2−/−/Il10−/− Mouse Model
Author(s)
Han, Qiyuan; Kono, Thomas J. Y.; Knutson, Charles G.; Parry, Nicola M.; Seiler, Christopher L.; Fox, James G.; Tannenbaum, Steven R.; Tretyakova, Natalia Y.; ... Show more Show less
Downloadijms-22-00364.pdf (2.184Mb)
Publisher with Creative Commons License
Publisher with Creative Commons License
Creative Commons Attribution
Terms of use
Metadata
Show full item recordAbstract
Epigenetic dysregulation is hypothesized to play a role in the observed association between inflammatory bowel disease (IBD) and colon tumor development. In the present work, DNA methylome, hydroxymethylome, and transcriptome analyses were conducted in proximal colon tissues harvested from the <i>Helicobacter hepaticus <i>(</i>H. hepaticus<i>)</i></i>-infected murine model of IBD. Reduced representation bisulfite sequencing (RRBS) and oxidative RRBS (oxRRBS) analyses identified 1606 differentially methylated regions (DMR) and 3011 differentially hydroxymethylated regions (DhMR). These DMR/DhMR overlapped with genes that are associated with gastrointestinal disease, inflammatory disease, and cancer. RNA-seq revealed pronounced expression changes of a number of genes associated with inflammation and cancer. Several genes including <i>Duox2</i>, <i>Tgm2</i>, <i>Cdhr5</i>, and <i>Hk2</i> exhibited changes in both DNA methylation/hydroxymethylation and gene expression levels. Overall, our results suggest that chronic inflammation triggers changes in methylation and hydroxymethylation patterns in the genome, altering the expression of key tumorigenesis genes and potentially contributing to the initiation of colorectal cancer.
Date issued
2020-12Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Division of Comparative MedicineJournal
International Journal of Molecular Sciences
Publisher
MDPI AG
Citation
International Journal of Molecular Sciences 22 (1): 364 (2021)
Version: Final published version
ISSN
1422-0067