Single-cell measurement of plasmid copy number and promoter activity
Author(s)
Shao, Bin; Rammohan, Jayan; Anderson, Daniel A; Alperovich, Nina; Ross, David; Voigt, Christopher A; ... Show more Show less
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Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.
Date issued
2021-03Department
Massachusetts Institute of Technology. Synthetic Biology CenterJournal
Nature Communications
Publisher
Springer Science and Business Media LLC
ISSN
2041-1723