Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays

Author(s)
Nagarajan, Maxwell B; Tentori, Augusto M; Zhang, Wen Cai; Slack, Frank J; Doyle, Patrick S
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Abstract
© 2020, The Author(s). Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-rasLSL-G12D/+; p53fl/fl). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics.
Date issued
2020
URI
https://hdl.handle.net/1721.1/134617
Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Journal
Microsystems and Nanoengineering
Publisher
Springer Science and Business Media LLC
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