| dc.contributor.author | Nagarajan, Maxwell B | |
| dc.contributor.author | Tentori, Augusto M | |
| dc.contributor.author | Zhang, Wen Cai | |
| dc.contributor.author | Slack, Frank J | |
| dc.contributor.author | Doyle, Patrick S | |
| dc.date.accessioned | 2021-10-27T20:05:47Z | |
| dc.date.available | 2021-10-27T20:05:47Z | |
| dc.date.issued | 2020 | |
| dc.identifier.uri | https://hdl.handle.net/1721.1/134617 | |
| dc.description.abstract | © 2020, The Author(s). Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-rasLSL-G12D/+; p53fl/fl). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics. | |
| dc.language.iso | en | |
| dc.publisher | Springer Science and Business Media LLC | |
| dc.relation.isversionof | 10.1038/S41378-020-0169-8 | |
| dc.rights | Creative Commons Attribution 4.0 International license | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.source | Nature | |
| dc.title | Spatially resolved and multiplexed MicroRNA quantification from tissue using nanoliter well arrays | |
| dc.type | Article | |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | |
| dc.relation.journal | Microsystems and Nanoengineering | |
| dc.eprint.version | Final published version | |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | |
| dc.date.updated | 2021-06-09T18:10:02Z | |
| dspace.orderedauthors | Nagarajan, MB; Tentori, AM; Zhang, WC; Slack, FJ; Doyle, PS | |
| dspace.date.submission | 2021-06-09T18:10:03Z | |
| mit.journal.volume | 6 | |
| mit.journal.issue | 1 | |
| mit.license | PUBLISHER_CC | |
| mit.metadata.status | Authority Work and Publication Information Needed | |
