Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy
Author(s)Yamauchi, Toyohiko; Sugiyama, Norikazu; Sakurai, Takashi; Iwai, Hidenao; Yamashita, Yutaka
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Cell membrane motions of living cells are quantitatively measured in nanometer resolution by low-coherent full-field quantitative phase microscopy. Our setup is based on a full-field phase shifting interference microscope with a very lowcoherent light source. The reflection mode configuration and the low-coherent illumination make it possible to differentiate the weak reflection light from the cell membrane from the strong reflection from the glass substrate. Two cell populations are quantitatively assessed by the power spectral density of the cell surface motion and show different trends.
DepartmentMassachusetts Institute of Technology. Spectroscopy Laboratory
Proceedings of SPIE--the International Society for Optical Engineering ; v. 7570
Yamauchi, Toyohiko et al. “Label-free classification of cell types by imaging of cell membrane fluctuations using low-coherent full-field quantitative phase microscopy.” Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII. Ed. Jose-Angel Conchello et al. San Francisco, California, USA: SPIE, 2010. 75700X-8. ©2010 SPIE.
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