Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy
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Second harmonic generation (SHG) microscopy has become an important tool for minimally invasive biomedical imaging. However, differentiation of different second harmonic generating species is mainly provided by morphological features. Using excitation polarization-resolved SHG microscopy we determined the ratios of the second-order susceptibility tensor elements at single pixel resolution. Mapping the resultant ratios for each pixel onto an image provides additional contrast for the differentiation of different sources of SHG. We demonstrate this technique by imaging collagen-muscle junction of chicken wing.
Date issued
2010-02Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical EngineeringJournal
Proceedings of SPIE--the International Society for Optical Engineering; v. 7569
Publisher
SPIE
Citation
Wei-Liang Chen, Tsung-Hsian Li, Ping-Jung Su, Chen-Kuan Chou, Peter Tramyeon Fwu, Sung-Jan Lin, Daekeun Kim, Peter T. C. So, and Chen-Yuan Dong (2010). Second-order susceptibility imaging with polarization-resolved second harmonic generation microscopy. Proc. SPIE 7569, 75691P/1-7. ©2010 COPYRIGHT SPIE--The International Society for Optical Engineering
Version: Final published version
ISSN
0277-786X
Keywords
second harmonic generation, polarization, microscopy, collagen